Biology

What Is Fehling’s Test? The Fehling’s test was developed by German chemist Hermann von Fehling in 1849. It is generally a test for reducing sugars and non-reducing sugars; and also a supplementary to the Tollens’ reagent test. The test can also be used to differentiate between water-soluble carbohydrate and ketone functional groups. Objectives of Fehling’s Test To detect the presence of carbohydrates in a solution. To … Read more

What Is Biuret Test? Biuret test is a chemical test used for detecting the presence of peptide bonds in a given analyte. Biuret test is also referred to as Piotrowski’s test, the name of Gustaw Piotrowski, a polish physiologist who first devised and explained the test in 1857.  In presence of peptide bonds, a copper … Read more

What Is Ninhydrin Test? The ninhydrin test is a chemical test which is used to check whether a given analyte contains amines or alpha-amino acids. In this test, the main reactant is ninhydrin which is a hydrocarbon with a chemical compound with the formula C9H6O4. By IUPAC nomenclature standards name, ninhydrin is also referred to … Read more

What Is Pauly’s Test? Pauly’s test is a chemical test to detect the amino acid histidine and tyrosine, this test is named after the German chemist Hermann Pauly, who first described it. In this test, when proteins containing either tyrosine or histidine are reacted with diazotized sulfanilic acid under alkaline conditions to form dark red … Read more

What Is Anthrone Test? Anthrone is a tricyclic sweet-smelling ketone. It is utilized for a well-known cellulose measure and in the colorimetric determination of carbohydrates. Anthrone test is used for qualitative and quantitative estimation of polysaccharides as well as monosaccharides. In this test, carbohydrates are dehydrated with concentrated sulphuric acid (H2SO4) to form ‘’furfural’’, which … Read more

What Is Bial’s Test? Bial’s test named after a German physician Manfred Bial, is a chemical test for detection of presence of pentoses. Bial’s test reagent contains concentrated Hydrochloric acid (HCl) as a dehydrating acid, orcinol and traces of ferric chloride as condensation reagent. A pentose if present in a given sample, will be dehydrated … Read more

What Is Seliwanoff’s Test? Seliwanoff’s test is a chemical test which differentiates between Aldose and ketose sugars. If the sugar contains a ketone group, it is a ketose whereas if it contains an aldehyde group, it is an Aldose. This test is much like Bial’s test; it relies on the principle that, when heated, ketoses … Read more

Microscope Description A microscope is a laboratory instrument used to examine objects that are too small to be seen by the naked eye. In other words, it enlarges images of small objects. Invented by a Dutch spectacle maker in the late 16th century, light microscopes use lenses and light to magnify images.  Generally a microscope … Read more

What Is Nitroprusside Test? Nitroprusside test is a biochemical test used for the detection of the free-SH groups in amino acids or the cysteine amino acid in a protein. This test uses sodium Nitroprusside reagents and ammonia solution. Nitroprusside is a complex anion consisting of an octahedral ferrous center which is surrounded by five tightly … Read more