Biochemical Test of Clostridium difficile

By Prof Mariam Mirambo Basic Characteristics Properties (Clostridium difficile) Capsule Capsulated Catalase Negative (-ve) Flagella Flagellated Gelatin Hydrolysis Positive (+ve) Gram Staining Positive (+ve) H2S Positive (+ve) Hemolysis Negative (-ve) Indole Negative (-ve) Motility Motile Nitrate Reduction Negative (-ve) OF (Oxidative-Fermentative) Anaerobic Oxidase Negative (-ve) Shape Rods Spore Positive (+ve) Urease Negative (-ve) VP (Voges … Read more

Biochemical Test of Campylobacter jejuni

By Prof Mariam Mirambo Basic Characteristics Properties (Campylobacter jejuni) Capsule Capsulated Catalase Positive (+ve) Flagella Flagellated Gram Staining Negative (-ve) Growth at 25°C Negative (-ve) Growth at 37°C Positive (+ve) Growth at 42°C Positive (+ve) Growth on MacConkey Agar Positive (+ve) H2S Negative (-ve) Hemolysis Non-Hemolytic KOH Positive (+ve) Motility Motile Nitrate Reduction Positive (+ve) Nitrite … Read more

Biochemical Test of Campylobacter fetus subsp. fetus

By Prof Mariam Mirambo Basic Characteristics Properties (Campylobacter fetus subsp. fetus) Capsule Capsule like surface (S-layer) proteins Catalase Positive (+ve) Gram Staining Gram-negative KMnO4 Positive (+ve) H2S Negative (-ve) Hemolysis Negative (-ve) Motility Positive (+ve) 2% NaCl Positive (+ve) Nitrate Reduction Positive (+ve) 2% Ox-bile Positive (+ve) Oxidase Positive (+ve) 0.02% Safranin Positive (+ve) Shape Slender curved rods; … Read more

Biochemical Test of Burkholderia pseudomallei

By Prof Miriam Mirambo Basic Characteristics Properties (Burkholderia pseudomallei) Capsule Positive (+ve) Catalase Positive (+ve) Citrate Positive (+ve) Flagella Positive (+ve) Gas Negative (-ve) Gelatin Hydrolysis Positive (+ve) Gram Staining Gram-negative (-ve) H2S Negative (-ve) Indole Negative (-ve) Motility Positive (+ve) Nitrate Reduction Positive (+ve) Oxidase Positive (+ve) Pigment Negative (-ve) Shape Rod-shaped; “safety pin” … Read more

Biochemical Test of Burkholderia cepacia

By Prof Mariam Mirambo Basic Characteristics Properties (Burkholderia cepacia) Bacteriocin Positive (+ve) Capsule Positive (+ve) Catalase Positive (+ve) Citrate Positive (+ve) Flagella Positive (+ve) Gelatin Hydrolysis Variable Gram Staining Gram-stain-negative Hemolysis Negative (-ve) Motility Positive(+ve) Nitrate Reduction Negative (-ve) Oxidase Variable Pigment Positive (+ve) Shape                   Straight or curved rods Spore Negative (-ve) Urease Positive … Read more

Biochemical Test of Bordetella pertussis

By Prof Mariam Mirambo Basic Characteristics Properties (Bordetella pertussis) Capsule Positive (+ve) Catalase Positive (+ve) Citrate Negative (-ve) Flagella Negative (-ve) Gelatin Hydrolysis Negative (-ve) Gram Staining Gram-negative (-ve) Growth in nutrient broth Negative (-ve) Hemolysis Positive (+ve) Motility Negative (-ve) Nitrate Reduction Negative (-ve) Oxidase Positive(+ve) Pigment Negative (-ve) Shape Minute coccobacilli Spore Negative … Read more

Biochemical Test of Bifidobacterium bifidum

By Prof Mariam Mirambo Basic Characteristics Properties (Bifidobacterium bifidum) 2% Bile Salt Positive (+ve) Capsule Negative (-ve) Catalase Negative (-ve) Flagella Negative (-ve) Gas Negative (-ve) Gelatin Hydrolysis Negative (-ve) Gram Staining Gram-Positive (+ve) Indole Negative (-ve) Motility Negative (-ve) Nitrate Reduction Negative (-ve) OF (Oxidative-Fermentative) Fermentative Oxidase Negative (-ve) Pigment Negative (-ve) Shape Pleomorphic rods … Read more

Biochemical Test of Bacteroides fragilis

By Prof Mariam Mirambo Basic Characteristics Properties (Bacteroides fragilis) Capsule Positive (+ve) Catalase Positive (+ve) Flagella Negative (-ve) Gelatin Hydrolysis Weakly Positive (+ve) Gram Staining Gram-negative (-ve) Growth in Bile (20%) Positive (+ve) Hemolysis Negative (-ve) Indole Variable Motility Negative (-ve) Nitrate Reduction Negative (-ve) Oxidase Variable Pigment Negative (-ve) Shape Rod-shaped cells with rounded … Read more

Biochemical Test of Bacillus cereus

By Prof Moses Joloba Basic Characteristics Properties (Bacillus cereus) Catalase Positive (+ve) Citrate Positive (+ve) Gelatin Hydrolysis Negative (-ve) Gram Staining Positive (+ve) Growth in KCN Positive (+ve) Hemolysis Positive (+ve) Indole Negative (-ve) Motility Positive (+ve) MR (Methyl Red) Negative (-ve) Nitrate Reduction Variable Oxidase Negative (-ve) Pigment Negative (-ve) Shape Rods Spore Positive … Read more