Biochemical Test of Fusobacterium necrophorum

By Prof Walter Jaoko

Basic CharacteristicsProperties (Fusobacterium necrophorum)
Bile GrowthVariable
CapsuleNegative (-ve)
CatalaseNegative (-ve)
FlagellaNegative (-ve)
GasPositive (+ve)
Gelatin HydrolysisVariable
Gram StainingGram-negative bacteria
H2SPositive (+ve)
HemolysisPositive (+ve) (alpha- and beta-hemolysis may be observed)
IndolePositive (+ve)
Methylene Blue ReductionPositive (+ve)
MotilityNegative (-ve)
MR (Methyl Red)Negative (-ve)
Nitrate ReductionNegative (-ve)
OxidaseNegative (-ve)
ShapePleomorphic rod ranging from small, almost coccoid bodies to filaments, with parallel sides and blunt or tapering ends.
SporeNegative (-ve)
VP (Voges Proskauer)Negative (-ve)
Fermentation of
AdonitolNegative (-ve)
ArabinoseNegative (-ve)
CellobioseNegative (-ve)
DNasePositive (+ve)
DulcitolNegative (-ve)
FructoseVariable
GlucoseVariable
GlycerolNegative (-ve)
GlycogenNegative (-ve)
InositolNegative (-ve)
InulinNegative (-ve)
LactoseNegative (-ve)
MaltoseNegative (-ve)
MannitolNegative (-ve)
MannoseNegative (-ve)
RaffinoseNegative (-ve)
RhamnoseNegative (-ve)
RiboseNegative (-ve)
SorbitolNegative (-ve)
SucroseNegative (-ve)
Enzymatic Reactions
N-Acetyl-glucosaminidaseNegative (-ve)
Acid PhosphatasePositive (+ve)
Alkaline PhosphatasePositive (+ve)
Beta-LactamasePositive (+ve)
Casein HydrolysisPositive (+ve)
Esculin HydrolysisNegative (-ve)
β -GlucosidaseNegative (-ve)
LecithinaseNegative (-ve)
LipasePositive (+ve)
Lysine decarboxylaseNegative (-ve)
PhosphataseNegative (-ve)
TryptophanasePositive (+ve)