Biocheminsider What Is Millon’s Test? Millon’s test is an analytical test used for detection of presence of soluble proteins. Millon’s test is one of the qualitative methods to determine differences in types of proteins and amino acids, namely the type of phenolic amino or amino acids that have phenol groups such as tyrosine and it derivatives. … Read more
What Is Molisch’s Test? Molisch’s test is a sensitive chemical test, named after Austrian botanist Hans Molisch, used for the test of presence of carbohydrates, based on the dehydration of the carbohydrate by sulfuric acid or hydrochloric acid to produce an aldehyde, which condenses with two molecules of a phenol, resulting in violet ring. A … Read more
What Is Barfoed’s Test? Barfoed’s test is a biochemical test used to detect monosaccharide (reducing) sugars in solution. The technique was devised by a Swedish physician C. T. Barfoed (1815–1899). Barfoed’s reagent, a mixture of ethanoic (acetic) acid and copper(II) acetate, is added to the test solution and boiled. If any reducing sugars are present … Read more
In males, testosterone is responsible for many normal functions including growth and development of the genitals, muscles and bones. It also helps in normal sexual development (puberty) in boys. Testosterone levels in men can often decline steeply after the age of 50, leading to a condition referred to as andropause in which vigor and sexual … Read more
What Is Xanthoproteic test? Xanthoproteic test is a biochemical test for the detection of amino acids having benzene ring in them like phenylalanine, tyrosine and tryptophan (aromatic amino acids). In the Xanthoproteic reaction, the yellowish substance produced by heating protein with nitric acid is xanthoprotein, hence the name Xanthoproteic test. The test is a qualitative … Read more
Introduction Coagulase is a protein enzyme produced by several microorganisms that enables the conversion of fibrinogen to fibrin. In the laboratory, it is used to distinguish between different types of Staphylococcus isolates. Staphylococcus aureus is known to produce coagulase, which can clot plama into gel in tube or agglutinate cocci in slide. This test is useful in differentiating S.aureus from other coagulase-negative staphylococci. Most strains of S.aureus … Read more
Introduction A germ tube test is a diagnostic test in which a sample of fungal spores are suspended in animal serum and examined by microscopy for the detection of any germ tubes. It is particularly indicated for colonies of white or cream color on fungal culture, where a positive germ tube test is strongly indicative of Candida albicans. Germ tube formation was first reported by Reynolds … Read more
Introduction Voges–Proskauer commonly VP is a test used to detect acetoin in a bacterial broth culture. The test is performed by adding alpha-naphthol and potassium hydroxide to the Voges-Proskauer broth which has been inoculated with bacteria. A cherry red color indicates a positive result, while a yellow-brown color indicates a negative result.The test depends on the digestion of glucose to acetylmethylcarbinol. In the presence of oxygen and strong … Read more
Introduction Negative staining is one of electron staining techniques. In Negative staining technique, an acidic stain such as Nigrosin, India ink, Eosin or Congo red is used in which the bacterial culture or the specimen is mixed well and then spread over the Microscopic glass slide to form a thin smear.This technique leaves heavy metals … Read more