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BiocheminsiderWhat Is Tollens’ Test? Tollens’ test also referred to as silver-mirror test is a qualitative laboratory test used to distinguish between aldehyde and ketone. The test is named after a German chemist, Bernard Tollens who discovered the test. The test involves use of Tollens’ reagent which must be prepared immediately prior to its use as … Read more
What Is Fehling’s Test? The Fehling’s test was developed by German chemist Hermann von Fehling in 1849. It is generally a test for reducing sugars and non-reducing sugars; and also a supplementary to the Tollens’ reagent test. The test can also be used to differentiate between water-soluble carbohydrate and ketone functional groups. Objectives of Fehling’s Test To detect the presence of carbohydrates in a solution. To … Read more
What Is Biuret Test? Biuret test is a chemical test used for detecting the presence of peptide bonds in a given analyte. Biuret test is also referred to as Piotrowski’s test, the name of Gustaw Piotrowski, a polish physiologist who first devised and explained the test in 1857. In presence of peptide bonds, a copper … Read more
What Is Lead Sulfide Test? Lead sulfide test also referred to as lead acetate test is a biochemical test for the detection of amino acids like cysteine and cystine. The test is a specific test for the detection of amino acids containing sulfur, S-S group in cysteine and S-H group in cysteine. It is important … Read more
What Is Ninhydrin Test? The ninhydrin test is a chemical test which is used to check whether a given analyte contains amines or alpha-amino acids. In this test, the main reactant is ninhydrin which is a hydrocarbon with a chemical compound with the formula C9H6O4. By IUPAC nomenclature standards name, ninhydrin is also referred to … Read more
What Is Pauly’s Test? Pauly’s test is a chemical test to detect the amino acid histidine and tyrosine, this test is named after the German chemist Hermann Pauly, who first described it. In this test, when proteins containing either tyrosine or histidine are reacted with diazotized sulfanilic acid under alkaline conditions to form dark red … Read more
What Is Anthrone Test? Anthrone is a tricyclic sweet-smelling ketone. It is utilized for a well-known cellulose measure and in the colorimetric determination of carbohydrates. Anthrone test is used for qualitative and quantitative estimation of polysaccharides as well as monosaccharides. In this test, carbohydrates are dehydrated with concentrated sulphuric acid (H2SO4) to form ‘’furfural’’, which … Read more
What Is Bial’s Test? Bial’s test named after a German physician Manfred Bial, is a chemical test for detection of presence of pentoses. Bial’s test reagent contains concentrated Hydrochloric acid (HCl) as a dehydrating acid, orcinol and traces of ferric chloride as condensation reagent. A pentose if present in a given sample, will be dehydrated … Read more
What Is Seliwanoff’s Test? Seliwanoff’s test is a chemical test which differentiates between Aldose and ketose sugars. If the sugar contains a ketone group, it is a ketose whereas if it contains an aldehyde group, it is an Aldose. This test is much like Bial’s test; it relies on the principle that, when heated, ketoses … Read more