Biochemical Test of Haemophilus influenzae

By Prof Moses Joloba

Basic CharacteristicsProperties (Haemophilus influenzae)
CapsuleStrain-dependent
CatalasePositive (+ve)
FlagellaNegative (-ve)
GasNegative (-ve)
Gram StainingGram-negative
H2SNegative (-ve)
HemolysisNegative (-ve)
IndoleVariable
MotilityNegative (-ve)
Nitrate ReductionPositive (+ve)
Nitrite ReductionNegative (-ve)
OxidasePositive (+ve)
PigmentAbsent or yellowish
ShapeCoccobacilli or small regular rods
UreaseVariable
V-factor requirementPositive (+ve)
Fermentation of
AdonitolNegative (-ve)
ArabinoseNegative (-ve)
CellobioseNegative (-ve)
DulcitolNegative (-ve)
ErythritolNegative (-ve)
FructoseNegative (-ve)
GalactosePositive (+ve)
GlucosePositive (+ve)
GlycerolNegative (-ve)
InositolNegative (-ve)
InulinNegative (-ve)
LactoseNegative (-ve)
MaltosePositive (+ve)
MannitolNegative (-ve)
MannoseNegative (-ve)
MelibioseNegative (-ve)
RaffinoseNegative (-ve)
RhamnoseNegative (-ve)
RibosePositive (+ve)
SalicinNegative (-ve)
SorbitolNegative (-ve)
SorboseNegative (-ve)
SucroseNegative (-ve)
TrehaloseNegative (-ve)
XylosePositive (+ve)
Enzymatic Reactions
Alkaline PhosphatasePositive (+ve)
Arginine DihydrolaseNegative (-ve)
Esculin HydrolysisNegative (-ve)
FucosidaseNegative (-ve)
IgA1 proteasePositive (+ve)
Lysine decarboxylaseVariable
NeuraminidasePositive (+ve)
ONPG (β-galactosidase)Negative (-ve)
Ornithine DecarboxylaseVariable