Biocheminsider

Biochemical Test of Enterobacter aerogenes

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By Prof Moses Joloba Basic Characteristics Properties (Enterobacter aerogenes) Capsule Positive (+ve) Catalase Positive (+ve) Citrate Positive (+ve) Flagella Positive (+ve) Gas Positive (+ve) Gelatin Hydrolysis Negative (-ve) Gram Staining Gram-negative H2S Negative (-ve) Indole Negative (-ve) Motility Positive (+ve) MR (Methyl Red) Negative (-ve) Nitrate Reduction Positive (+ve) OF (Oxidative-Fermentative) Fermentative Oxidase Negative (-ve) … Read more

Biochemical Test of Pseudomonas aeruginosa

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Reviewed By Prof Moses Joloba Pseudomonas aeruginosa is a gram-negative, rod-shaped bacterium belonging to the genus Pseudomonas. It is known for its remarkable adaptability, intrinsic resistance to many antibiotics, and its ability to cause a wide range of infections, particularly in immunocompromised individuals. Pseudomonas aeruginosa appears as small, straight or slightly curved rods under the … Read more

Biochemical Test of Enterococcus faecalis

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By Prof Jeremiah Seni Basic Characteristics Properties (Enterococcus faecalis ) Bile Resistance Resistance to 40% (v/v) bile Capsule Negative (-ve) Catalase Negative (-ve) Citrate Negative (-ve) Flagella Negative (-ve) Gelatin Hydrolysis Variable Gram Staining Gram-positive H2S Negative (-ve) Hemolysis Negative (-ve) Indole Negative (-ve) Motility Negative (-ve) 6.5% NaCl Positive (+ve) OF (Oxidative-Fermentative) Fermentative Oxidase Negative … Read more

Biochemical Test of Serratia marcescens

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By Prof Jeremiah Seni Basic Characteristics Properties (Serratia marcescens) Capsule Negative (-ve) Catalase Positive (+ve) Citrate Positive (+ve) Flagella Positive (+ve) Gas Variable Gelatin Hydrolysis Positive (+ve) Gram Staining Negative (-ve) H2S Negative (-ve) Indole Negative (-ve) Motility Positive (+ve) MR (Methyl Red) Negative (-ve) Nitrate Reduction Positive (+ve) OF (Oxidative-Fermentative) Facultative Anaerobes Oxidase Negative … Read more

Biochemical Test of Gardnerella vaginalis

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By Prof Jeremiah Seni Basic Characteristics Properties (Gardnerella vaginalis) Capsule Negative (-ve) Catalase Negative (-ve) Flagella Negative (-ve) Gas Negative (-ve) Gelatin Hydrolysis Negative (-ve) Gram Staining Gram negative (-ve) or Gram variable H2S Negative (-ve) Hemolysis Positive (+ve) (Human or Rabbit Blood) Indole Negative (-ve) Motility Negative (-ve) MR (Methyl Red) Positive (+ve) Nitrate Reduction … Read more

Biochemical Test of Citrobacter freundii

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By Prof Jeremiah Seni Basic Characteristics Properties (Citrobacter freundii) Capsule Negative (-ve) Catalase Positive (+ve) Citrate Positive (+ve) Flagella Positive (+ve) Gas from glucose Positive (+ve) Gelatin Hydrolysis Negative (-ve) Gram Staining Negative (-ve) Growth in KCN Positive (+ve) H2S Positive (+ve) Indole Negative (-ve) Motility Positive (+ve) MR (Methyl Red) Positive (+ve) Nitrate Reduction … Read more

Biochemical Test of Brucella melitensis

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By Prof Jeremiah Seni Basic Characteristics Properties (B. melitensis) Capsule Negative (-ve) Catalase Positive(+ve) Flagella Negative (-ve) Gelatin Hydrolysis Negative (-ve) Gram Staining Gram Negative (-ve) H2S Negative (-ve) Hemolysis Negative (-ve) Indole Negative (-ve) Litmus Milk Negative (-ve) Motility Negative (-ve) MR (Methyl Red) Negative (-ve) Nitrate Reduction Positive(+ve) Oxidase Positive(+ve) Shape Coccobacilli, or … Read more

Nitrate Reduction Test: Objective, Principle, Procedure And Result Interpretation

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By Prof Walter Jaoko Introduction The nitrate reduction test is a qualitative procedure for determining the ability of bacteria to reduce nitrate. In the reaction, nitrate is reduced to nitrite, which may then be further reduced to nitrogen gas or ammonia.Other uses of Nitrate Reduction Tests are:  Differentiating Mycobacterium species. Identifying species of Neisseria and separating them from Moraxella and Kingella species. The nitrate … Read more

Acid Fast Stain: Principle, Reagents, Procedure And Result Interpretation

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By Prof Walter Jaoko Introduction Acid-Fast (AF) is an important special staining technique used in the histology laboratory. It is a differential staining techniques which was first developed by Ziehl and later on modified by Neelsen 1883. This method is used for those microorganisms which are not staining by simple or Gram staining method, particularly the member of … Read more