| Location | It is found freely in the central portion of the cytoplasm. | It is found within the nucleus. |
| Size | The size of DNA in a prokaryote is usually less than 0.1 pg. | The size of DNA in eukaryotes is high, usually more than 1 pg. |
| Chromosome | These do not form chromosomes. | They form chromosomes in the nucleus. |
| Number of Chromosomes | The prokaryotic DNA is organized into a single chromosome. | The eukaryotic DNA is organized into many chromosomes. |
| Occurrence | Is found occurring as a closed covalent circular form of DNA. | Occurs as a linear form of DNA with two ends. |
| Introns | Introns are absent in the coding region of prokaryotic DNA. | Introns are present in the coding region of eukaryotic DNA. |
| Nucleosome | There is no formation of nucleosomes in prokaryotic DNA. | Nucleosomes are formed in the DNA of eukaryotic cells. |
| Genomes | The genome of prokaryotes is too small and contains a few genes ranging from several kb to MB in size. | The eukaryotic genome is usually larger contains a few thousand genes (~21,000 genes in humans) and is made up of billions of base pairs. |
| Quantity of the DNA | The quantity of the DNA is less as compared to that in eukaryotes. | The quantity of the DNA is more. |
| DNA Replication | DNA Replication occurs in the cytoplasm of the cell. | DNA Replication is found occurring in the nucleus of the cell. |
| Transposons | The prokaryotic DNA does not consist of transposons. | The eukaryotic DNA consists of transposons. |
| Histone Proteins | This DNA does not interact or associate with histone proteins. | They interact with histone proteins. |
| Plasmid DNA | Prokaryotes possess a smaller, circular, single-stranded DNA, known as the plasmid DNA. | Eukaryotes do not possess plasmid DNA. |