| Prokaryotic DNA Replication | Eukaryotic DNA Replication |
| Prokaryotic DNA replication happens in the cytoplasm of the cell. | The eukaryotic replication takes place in the nucleus of the cell. |
| Per DNA molecule, there exists only a single point of origin. | This DNA molecule consists of multiple points of origin on a single. |
| The origin is composed of about 100-200 or more nucleotides. | The origin of replication is constructed of roughly 150 nucleotides. |
| The replication process is bi-directional and only two replication forks are formed. | Several replication forks are formed in multiple replication beads. |
| The prokaryotic chromosome contains only one replicon. | The eukaryotic chromosome contains around 50,000 replicons. |
| The procedure is carried out by DNA Polymerase I and III. | The procedure is carried out by the DNA Polymerase α, δ, and ε |
| It requires DNA gyrase | It doesn’t require DNA gyrase. |
| The Okazaki fragments are large. They are approximately 1000-2000 nucleotides in length. | The Okazaki fragments are small and are roughly 100-200 nucleotides in length. |
| It is a quick approach with nearly 2000 nucleotides added per second. | It is a slow approach having around 100 nucleotides added per second. |
| It includes circular and double-stranded DNA. | It includes linear and double-stranded DNA. |