Biochemical Test of Clostridium tetani

Basic CharacteristicsProperties (Clostridium tetani)
CapsuleNegative (-ve)
CatalaseNegative (-ve)
FlagellaPeritrichous
GasPositive (+ve)
Gelatin HydrolysisPositive (+ve)
Gram StainingGram-stain-positive, but become Gram-stain-negative after approximately 24 h incubation.
Growth in 20% BileNegative (-ve)
Growth in 6.5% NaClNegative (-ve)
H2SPositive (+ve)
HemolysisPositive (+ve)
IndoleVariable
MotilityPositive (+ve)
Nitrate ReductionNegative (-ve)
ShapeLarge rods (0.5-1.7 x 2.1-18.1 ┬Ám), which may appear single, in pairs or in short chains.
SporePositive (+ve)
Fermentation of
ArabinoseNegative (-ve)
CellobioseNegative (-ve)
DNasePositive (+ve)
FructoseNegative (-ve)
GalactoseNegative (-ve)
GlucoseNegative (-ve)
GlycogenNegative (-ve)
InositolNegative (-ve)
InulinNegative (-ve)
LactoseNegative (-ve)
MaltoseNegative (-ve)
MannitolNegative (-ve)
MannoseNegative (-ve)
RaffinoseNegative (-ve)
RhamnoseNegative (-ve)
RiboseNegative (-ve)
SalicinNegative (-ve)
SorbitolNegative (-ve)
StarchNegative (-ve)
SucroseNegative (-ve)
TrehaloseNegative (-ve)
XyloseNegative (-ve)
Enzymatic Reactions
Esculin HydrolysisNegative (-ve)
LecithinaseNegative (-ve)
LipaseNegative (-ve)
NeuraminidaseNegative (-ve)
Superoxide dismutase Negative (-