| Basic Characteristics | Properties (Clostridium perfringens) |
| 20% Bile | Positive (+ve) |
| Capsule | Positive (+ve) |
| Catalase | Negative (-ve) |
| Flagella | Negative (-ve) |
| Gas | Positive (+ve) |
| Gelatin Hydrolysis | Positive (+ve) |
| Gram Staining | Gram-positive |
| Hemolysis | Positive (+ve) |
| Indole | Negative (-ve) |
| Motility | Negative (-ve) |
| Nitrate Reduction | Variable |
| Oxidase | Negative (-ve) |
| Shape | Rod shaped; straight rods with blunt ends |
| Spore | Positive (+ve) |
| 2 % NaCl | Positive (+ve) |
| 6.5% NaCl | Negative (-ve) |
| Arabinose | Negative (-ve) |
| Cellobiose | Variable |
| DNase | Positive (+ve) |
| Fructose | Positive (+ve) |
| Galactose | Positive (+ve) |
| Glucose | Positive (+ve) |
| Glycerol | Weakly Positive (+ve) |
| Glycogen | Variable |
| Inositol | Variable |
| Inulin | Negative (-ve) |
| Lactose | Positive (+ve) |
| Maltose | Positive (+ve) |
| Mannitol | Positive (+ve) |
| Mannose | Positive (+ve) |
| Melibiose | Negative (-ve) |
| Raffinose | Variable |
| Ribose | Variable |
| Salicin | Negative (-ve) |
| Sorbitol | Negative (-ve) |
| Starch | Variable |
| Sucrose | Positive (+ve) |
| Trehalose | Variable |
| Xylose | Negative (-ve) |
| Amylase | Positive (+ve) |
| Elastase | Positive (+ve) |
| Esculin Hydrolysis | Variable |
| Hyalurodinase | Positive (+ve) |
| Lecithinase | Positive (+ve) |
| Lipase | Negative (-ve) |
| Neuraminidase | Positive (+ve) |
| ONPG (β-galactosidase) | Positive (+ve) |
| Ribonuclease | Positive (+ve) |
| Superoxide dismutase | Positive (+ve) |