| Basic Characteristics | Properties (Bifidobacterium bifidum) |
| 2% Bile Salt | Positive (+ve) |
| Capsule | Negative (-ve) |
| Catalase | Negative (-ve) |
| Flagella | Negative (-ve) |
| Gas | Negative (-ve) |
| Gelatin Hydrolysis | Negative (-ve) |
| Gram Staining | Gram-Positive (+ve) |
| Indole | Negative (-ve) |
| Motility | Negative (-ve) |
| Nitrate Reduction | Negative (-ve) |
| OF (Oxidative-Fermentative) | Fermentative |
| Oxidase | Negative (-ve) |
| Pigment | Negative (-ve) |
| Shape | Pleomorphic rods |
| Spore | Negative (-ve) |
| Amylose | Variable |
| Cellobiose | Negative (-ve) |
| Fructose-6-Phosphate | Positive (+ve) |
| Galactose | Positive (+ve) |
| Glucose | Positive (+ve) |
| Inulin | Negative (-ve) |
| Lactose | Positive (+ve) |
| Maltase | Positive (+ve) |
| Maltose | Variable |
| Mannitol | Negative (-ve) |
| Mannose | Negative (-ve) |
| Melibiose | Variable |
| Pectin | Negative (-ve) |
| Raffinose | Negative (-ve) |
| Ribose | Negative (-ve) |
| Salicin | Negative (-ve) |
| Sorbitol | Negative (-ve) |
| Starch | Negative (-ve) |
| Sucrose | Variable |
| Trehalose | Negative (-ve) |
| Xylan | Negative (-ve) |
| Xylose | Negative (-ve) |
| Arabinosidases | Positive (+ve) |
| Fructose 6-phosphoketolase | Positive (+ve) |
| Glucosidases | Positive (+ve) |
| Glutamate dehydrogenase | Positive (+ve) |
| Glutamine synthetase | Positive (+ve) |
| Hexosaminidases | Positive (+ve) |
| ONPG (β-galactosidase) | Positive (+ve) |